New genetic-labeling method uses a single gene to reveal neuronal circuits from multiple upstream regions
Scientists develop a method to genetically label neurons with a single gene of interest in mice by combining the anterograde transsynaptic spread of adeno-associated virus serotype 1 (AAV1) with intersectional gene expression. In two distinct circuits: the retina/primary visual cortex to the superior colliculus and the bilateral motor cortex to the dorsal striatum, injections of AAV1 expressing either Cre or Flpo recombinases and the Cre/Flpo double-dependent AAV into two upstream regions and the downstream region, respectively, were used to label postsynaptic neurons receiving inputs from the two upstream regions.
from Latest Science News -- ScienceDaily https://ift.tt/5WDCYAz
from Latest Science News -- ScienceDaily https://ift.tt/5WDCYAz
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